The purpose of this experiment is to use polymerase chain reaction and electrophoresis to analyze polymorphisms between students at the alu insertion in chromosome 16 pv92. Each section contains 1422 lab exercises, with instructor notes in appendices as well as an answer guide as a part of the book companion site. Making solutions and buffer efficacy, 15 4 mb experiment 4. Alu human dna typing your students use primers for a 300 base pair alu insertion in chromosome 16 pv92 to determine their own genotype.
The use of polymorphic alu insertions in human dna fingerprinting. Multiplex pcrbased alu insertion polymorphisms genotyping for identifying individuals of japanese ethnicity. Safe experiment for classroom use to demonstrate dna fingerprinting. Pcr based alu human dna typing, 45 11 mb experiment 11. Our dna fingerprinting procedure using pcr amplification of diallelic. Methods in biotechnology by seungbeom hong, 9781119156789, available at book depository with free delivery worldwide. Pcrbased aluhuman dna typing kit school science equipment. Aluhuman dna typing using pcr person possesses an alu insertion at the pv92 locus. This lab is a modification of an exercise developed by edvotek edvotek, 2003.
Alu insertions are widely dispersed in the human genome, and several databases. Results page 8 about pcr based alu human dna typing free essays. They can then compare their class results with others around the world over the. Pcrbased aluhuman dna typing by jessie macdonald on prezi. Alu human dna typing through pcr abstract this experiment is a qualitative experiment which shows if an individual has a specific dimorphic alu element. Pcr based alu human dna typing 45 11 mb experiment 11.
Multiplex pcrbased alu insertion polymorphisms genotyping for. Nonmammalian genomic analysis a practical guide this page intentionally left blank nonmammalian genomic analysis a. They are not to be used for diagnostic or drug purposes, nor administered to or consumed by humans. Two of the most recently formed subfamilies of alu elements within the human genome have been termed ya5 and. The epub format uses ebook readers, which have several ease of reading features. It amplifies the dna gather so clear bands can be form during gel electrophoresis. First, they are typed by rapid, simple, pcrbased assays.
Lab 2 pcrbased aluhuman dna typing aron wardhaugh purpose. May 10, 2012 uses less space while delivering ansi required 15 minute flush clear tank allows easy fill level check three mounting options. Restriction enzyme digestion, 51 12 mb experiment 12. View notes alu human pcr experimental procedure from bi 281 at carroll college. Methods in biotechnology is an invaluable resource for those students and professionals. Buy a discounted paperback of methods in biotechnology online from australias leading online bookstore.
Pcr based alu human dna typing, 45 11 mb experiment. Tap reaction tube to assure the reaction pellet is at the bottom of tube. Therefore, rapid genotyping can be performed in routine testing by subsequent capillary electrophoresis. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Alu insertion polymorphisms polymorphisms consisting of the presenceabsence of an alu element at a particular chromosomal location offer several advantages over other nuclear dna polymorphisms for human evolution studies. Background on alu goal noncoding sequence name for alu 1 recognition site selfreplicating selfish places itself back into the genome can result in disease to isolated and amplify dna with pcr run through electrophoresis examine the banding patterns one bp band. This chapter discusses the first twolaboratory analysis and pattern comparisonand chapter 3 focuses on statistical analysis. One of the most serious concerns regarding pcrbased typing is contamination of evidence samples with other human dna. C4n6, c4n2, c4n5, c4n8 based on studies of caucasian, asian, american black and.
Pcr based alu human dna typing experimental protocol experimentobjective. Edvotek pcrbased dna typing aluhuman pcrbased dna typing. Preface, vii acknowledgements, ix introduction to biotechnology lab, xi about the companion website, xix part i. Lane 5 was dna ladder, lane 6 had my dna and lane 7 had mirnas dna after it went through pcr for 30 cycles with pv92 primer. Roles of dna and rna in the human body and medicine anatomy and physiology 1 dr. Single nucleotides are then added opposite their complementary. Lane 2 and 3 which are 15 and 30 pcr cycles respectively show that the 30 pcr cycle produced more dna than 15 cycle, indicated by the more intense band in lane 3. Before any particular dna typing method is used for forensic purposes, it is essential that precise and scientifically reliable procedures be established for performing all three steps. Use of the spectrophotometer and beers law, 9 3 mb experiment 3. Aluhuman dna typing through pcr abstract this experiment is a qualitative experiment which shows if an individual has a specific dimorphic alu element. If a person is homozygous for the insertion, a gel of the pcr product will result in a single band at 700 base pairs figure 1a. Methods in biotechnology by seungbeom hong 9781119156789. Introduction knowing whether or not an individual possesses a certain gene. This text is broken into three sections based on level methods in biotechnology, advanced methods in biotechnology i, and advanced methods in biotechnology ii.
Potentially, amplification of contaminant dna could lead to spurious typing results. Oh group in order to fit onto the dna strand and then to add the next nucleotide. Pcr based aluhuman dna typing kit educational materials biology educational materials pcr learning activities using their own hair or cheek cells as sources of dna, students will isolate human dna and compare dna polymorphisms between individuals. Biotechnology classroom dna, rna, and pcr applications. We offer pcr based alu human dna typing kits at great prices with free delivery for uk schools. Lab 16 human dna typing using pcr written by eilene lyons. The use of dimorphic alu insertions in human dna fingerprinting. Alu human dna typing by maddalena zuccarello on prezi.
Pcr is not discriminating as to the source of the dna it amplifies, and it can be exceedingly sensitive. Klappentext as rapid advances in biotechnology occur, there is a need for a pedagogical tool to aid current students and laboratory professionals in biotechnological methods. Methods in biotechnology engages the reader by implementing an active learning approach, provided advanced study questions, as well. Aluhuman dna typing your students use primers for a 300 base pair alu insertion in chromosome 16 pv92 to determine their own genotype. Testing plant substances for antimicrobial activity, 63. Pcrbased aluhuman dna typing 45 11 mb experiment 11. Edvotek pcrbased dna typing aluhuman pcrbased dna typing teaching supplies. Booktopia has methods in biotechnology by seungbeom hong. Stanford libraries official online search tool for books, media, journals, databases, government documents and more. Your students use primers for a 300 base pair alu insertion in chromosome 16 pv92 to determine their own genotype. Wall, bench top or transportable waste cart allows for a fluid startstop flow with no expensive cartridges to replace water preservative sold separately.
Uses less space while delivering ansi required 15 minute flush clear tank allows easy fill level check three mounting options. View notes aluhuman pcr experimental procedure from bi 281 at carroll college. Pcr based alu human dna typing aron wardhaugh purpose. It is a very interesting experiment designed to isolate human dna and compare dna polymorphisms between individuals.
Lab 15 pcr based alu human dna typing figure 1 gel electrophoresis of 0. Joy henry schonathan crews 3202015 roles of dna and rna in the human body and medicine introduction deoxyribonucleic acid dna is the foreman of the body with a strict set of blueprints for what needs to be done in an organisms cell and how. Pcr based aluhuman dna typing experimental protocol experimentobjective. Methods in biotechnology mb laboratory exercises 1 1 mb experiment 1. We used a process called polymerase chain reaction pcr to identify this alu element. Pcrbased aluhuman dna typing, 45 11 mb experiment 11. Transfer 10 ul of the dna supernatant to pcr reaction tube while trying to avoid the pellet and chelating agent. They can then compare their class results with others around the world over the internet. An interactive dna investigationselect from an extensive catalog of educational tools available for enhancing the teaching of biological subjects, including simulated testing of blood and urine, the illustration of various natural habitats, and the dissection of sterilized stool samples collected from different regions. We have characterized several human specific hs alu insertions as either. Part of the progress in systems and control theory book series exs. Your students use primers for a 300 base pair alu insertion in chromosome 16 pv92 to determine their. Spin tube in microcentrofuge for 2 mins to pellet cell debris leaving dna in supernatant 1518. Pcr based alu human dna typing kit educational materials biology educational materials pcr learning activities using their own hair or cheek cells as sources of dna, students will isolate human dna and compare dna polymorphisms between individuals.
Pcr based aluhuman dna typing, dolly, and epigenetics. Testing plant substances for antimicrobial activity 63. Label the tube containing the pcr reaction with initials on top. Access free textbook solutions and ask 5 free questions to expert tutors 247. Ouchterlony and elisa immunoassays, 57 14 mb experiment 14. The amount of dna produced after 15 cycles and 30 cycles was compared using 0. Background on alu goal noncoding sequence name for alu1 recognition site selfreplicating selfish places itself back into the genome can result in disease to isolated and amplify dna with pcr run through electrophoresis examine the banding patterns one bp band. Pcrbased aluhuman dna typing adapted from fbi technology. Experiment 11 pcr and dna typing flashcards quizlet.
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